Discovery of 1,5-Dihydro-4H-imidazol-4-one Derivatives as Potent, Selective Antagonists of CXC Chemokine Receptor 2.

Abstract

CXC chemokine receptors 1 (CXCR1) and 2 (CXCR2) have been demonstrated to have critical roles in cancer metastasis. Because they share high homology sequences, it is still unclear how to design selective CXCR1 or CXCR2 antagonists. Based on a pharmacophore model we built, compound 2 bearing a 1,5-dihydro-4H-imidazol-4-one scaffold was identified as a selective CXCR2 antagonist with a low CXCR1 antagonism preference. Further optimization and structure-activity relationship studies led to compound C5 that overcame the disadvantages of compound 2 and performed with higher selectivity. It showed excellent oral bioavailability and in vitro anticancer metastasis activity. Further dynamic simulation of the molecular protein complex showed that the amino acid residue K320 of CXCR2 contributed most to the selectivity of C5. This study provides important clues for the design of new CXCR2 selective antagonists, and C5 can be a molecular tool for investigating the difference in the biological function of CXCR1 and CXCR2.

• Authors: Jinxin Che, Zhilong Wang, Zheyuan Shen, Weihao Zhuang, Huazhou Ying, Yongzhou Hu, Youhong Hu, Xin Xie, Xiaowu Dong
• Journal: ACS medicinal chemistry letters (ACS Med Chem Lett) Vol. 12 Issue 5 Pg. 836-845 (May 13 2021) ISSN: 1948-5875 [Print] United States
• PMID: 34055234 (Publication Type: Journal Article)
• Copyright: © 2021 American Chemical Society.