Isolation of DNA sequences on human chromosome 21 by application of a recombination-based assay to DNA from flow-sorted chromosomes.

Abstract

By merging two efficient technologies, bivariate flow sorting of human metaphase chromosomes and a recombination-based assay for sequence complexity, we isolated 28 cloned DNA segments homologous to loci on human chromosome 21. Subregional mapping of these DNA segments with a somatic cell hybrid panel showed that 26 of the 28 cloned DNA sequences are distributed along the long arm of chromosome 21, while the other 2 hybridize with sequences on the short arm of both chromosome 21 and other chromosomes. This new collection of probes homologous to chromosome 21 should facilitate molecular analyses of trisomy 21 by providing DNA probes for the linkage map of chromosome 21, for studies of nondisjunction, for chromosome walking in clinically relevant subregions of chromosome 21, and for the isolation of genes on chromosome 21 following the screening of cDNA libraries.

• Authors: U Tantravahi, G D Stewart, M Van Keuren, G McNeil, S Roy, D Patterson, H Drabkin, M Lalande, D M Kurnit, S A Latt
• Journal: Human genetics (Hum Genet) Vol. 79 Issue 3 Pg. 196-202 (Jul 1988) ISSN: 0340-6717 [Print] Germany
• PMID: 3402991 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Recombinant
• DNA

Topics

• Animals
• Cell Line
• Chromosomes, Human, Pair 21
• Cloning, Molecular
• DNA (genetics, isolation & purification)
• DNA, Recombinant (analysis)
• Flow Cytometry
• Humans
• Hybrid Cells (cytology)
• Nucleic Acid Hybridization
• Plasmids