Plastrum testudinis extract suppresses osteoclast differentiation via the NF-κB signaling pathway and ameliorates senile osteoporosis.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Plastrum testudinis (PT) is a kind of single traditional Chinese medicine that can tonify kidney and strengthen bone. Plastrum testudinis extract (PTE) has been approved to promote the osteogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro. However, the mechanism by which PTE reduces osteoclast differentiation has not yet been reported.

AIM OF THE STUDY:

To explore the potential of PTE as a therapeutic treatment for bone loss caused by senile osteoporosis (SOP).

MATERIALS AND METHODS:

We evaluated whether PTE could inhibit RANKL-induced osteoclast differentiation both in vitro and in vivo, and investigated PTE-induced phenotypes of human peripheral blood monocytes.

RESULTS:

We found that PTE inhibited osteoclast differentiation and bone resorption in vitro in a concentration-dependent manner and that PTE treatment is most effective during the early stages of osteoclastogenesis. Moreover, we found that PTE could block the NF-κB signaling pathway in vitro, leading to the down-regulation of osteoclast-specific genes including C-FOS and NFATC1. The results from our in vivo mouse study suggest that PTE treatment suppresses osteoclast formation and mitigates bone loss caused by SOP. Notably, we also found that PTE inhibited RANKL-induced osteoclast differentiation in human peripheral blood monocytes.

CONCLUSION:

Our results suggest that PTE treatment suppresses osteoclastogenesis and ameliorates bone loss caused by SOP by selectively blocking the nuclear translocation of NF-κB/p50.

• Authors: Honglin Chen, Gengyang Shen, Qi Shang, Peng Zhang, Die Yu, Xiang Yu, Zhida Zhang, Wenhua Zhao, Zixian Wu, Fuyu Tang, De Liang, Xiaobing Jiang, Hui Ren
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 276 Pg. 114195 (Aug 10 2021) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 33974944 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Lipopolysaccharides
• NF-kappa B
• NF-kappa B p50 Subunit
• NFATC Transcription Factors
• RANK Ligand
• Tissue Extracts
• plastrum testudinis

Topics

• Animals
• Bone Resorption (chemically induced, drug therapy, metabolism)
• Cell Differentiation (drug effects)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Female
• Humans
• Lipopolysaccharides (toxicity)
• Male
• Mice, Inbred C57BL
• NF-kappa B (metabolism)
• NF-kappa B p50 Subunit (metabolism)
• NFATC Transcription Factors (metabolism)
• Osteoclasts (cytology, drug effects, metabolism)
• Osteogenesis (drug effects)
• Osteoporosis (drug therapy, etiology, metabolism)
• RANK Ligand (toxicity)
• Signal Transduction (drug effects)
• Tissue Extracts (pharmacology, therapeutic use)
• Mice