Prodigiosin of Serratia marcescens ZPG19 Alters the Gut Microbiota Composition of Kunming Mice.

Abstract

Prodigiosin is a red pigment produced by Serratia marcescens with anticancer, antimalarial, and antibacterial effects. In this study, we extracted and identified a red pigment from a culture of S. marcescens strain ZPG19 and investigated its effect on the growth performance and intestinal microbiota of Kunming mice. High-performance liquid chromatography/mass spectrometry revealed that the pigment had a mass-to-charge ratio (m/z) of 324.2160, and thus it was identified as prodigiosin. To investigate the effect of prodigiosin on the intestinal microbiota, mice (n = 5) were administered 150 μg/kg/d prodigiosin (crude extract, 95% purity) via the drinking water for 18 days. Administration of prodigiosin did not cause toxicity in mice. High-throughput sequencing analysis revealed that prodigiosin altered the cecum microbiota abundance and diversity; the relative abundance of Desulfovibrio significantly decreased, whereas Lactobacillus reuteri significantly increased. This finding indicates that oral administration of prodigiosin has a beneficial effect on the intestinal microbiota of mice. As prodigiosin is non-toxic to mouse internal organs and improves the mouse intestinal microbiota, we suggest that it is a promising candidate drug to treat intestinal inflammation.

• Authors: Xue Li, Xinfeng Tan, Qingshuang Chen, Xiaoling Zhu, Jing Zhang, Jie Zhang, Baolei Jia
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 26 Issue 8 (Apr 09 2021) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 33918541 (Publication Type: Journal Article)

Chemical References

• Prodigiosin

Topics

• Animals
• Biodiversity
• Body Weight (drug effects)
• Fermentation (drug effects)
• Gastrointestinal Microbiome (drug effects)
• Metabolome
• Mice
• Organ Specificity (drug effects)
• Phenotype
• Phylogeny
• Prodigiosin (chemistry, pharmacology)
• Serratia marcescens (chemistry, metabolism)