Abstract |
Lack of detailed knowledge of SARS-CoV-2 infection has been hampering the development of treatments for coronavirus disease 2019 (COVID-19). Here, we report that RNA triggers the liquid-liquid phase separation (LLPS) of the SARS-CoV-2 nucleocapsid protein, N. By analyzing all 29 proteins of SARS-CoV-2, we find that only N is predicted as an LLPS protein. We further confirm the LLPS of N during SARS-CoV-2 infection. Among the 100,849 genome variants of SARS-CoV-2 in the GISAID database, we identify that ~37% (36,941) of the genomes contain a specific trio- nucleotide polymorphism (GGG-to-AAC) in the coding sequence of N, which leads to the amino acid substitutions, R203K/G204R. Interestingly, NR203K/G204R exhibits a higher propensity to undergo LLPS and a greater effect on IFN inhibition. By screening the chemicals known to interfere with N- RNA binding in other viruses, we find that (-)- gallocatechin gallate (GCG), a polyphenol from green tea, disrupts the LLPS of N and inhibits SARS-CoV-2 replication. Thus, our study reveals that targeting N- RNA condensation with GCG could be a potential treatment for COVID-19.
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Authors | Ming Zhao, Yu Yu, Li-Ming Sun, Jia-Qing Xing, Tingting Li, Yunkai Zhu, Miao Wang, Yin Yu, Wen Xue, Tian Xia, Hong Cai, Qiu-Ying Han, Xiaoyao Yin, Wei-Hua Li, Ai-Ling Li, Jiuwei Cui, Zhenghong Yuan, Rong Zhang, Tao Zhou, Xue-Min Zhang, Tao Li |
Journal | Nature communications
(Nat Commun)
Vol. 12
Issue 1
Pg. 2114
(04 09 2021)
ISSN: 2041-1723 [Electronic] England |
PMID | 33837182
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nucleocapsid Proteins
- RNA, Viral
- gallocatechin gallate
- Catechin
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Topics |
- Amino Acid Substitution
(drug effects)
- COVID-19
(prevention & control, virology)
- Catechin
(analogs & derivatives, pharmacology)
- Genome, Viral
(genetics)
- Humans
- Liquid-Liquid Extraction
- Nucleocapsid Proteins
(genetics, metabolism)
- RNA, Viral
(genetics, metabolism)
- SARS-CoV-2
(drug effects, genetics)
- Virus Replication
(drug effects, genetics)
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