Diabetic
wounds have a slow healing process and easy to be infected. In addition to current
drug treatments, supportive approaches are needed for diabetic
wound treatment. In this study, we aimed to load Aloe Vera (AV) and Hypericum perforatum oil (HPO) with PCL/Ge (Poly (ɛ-
caprolactone)/Gelatine) polymeric biodegradable by electrospinning method into nanofiber dressings on an experimental diabetic
wound model to compare the diabetic wound healing effect. Changes in the amount and chemical structure of
phospholipids,
proteins, and
lipids were investigated in the blood and serum samples of the animals using Fourier transform infrared (FTIR) analysis. To evaluate
biological events associated with the
wound repair process in inflammatory phase we used
oxidant and
antioxidant status to determine the healing status of
wounds such as Total
antioxidant status (TAS), Total
oxidant level (TOS) and
tumor necrosis factor alpha (TNF-α) levels. TOS level increased in DM groups and decreased in the AV and HPO group. Oxidative stress index decreased and TNF-α level increased in the HPO group. FTIR spectra showed changes in the
phospholipids,
proteins, and
carbon chain of
lipids in the whole blood as well as serum of DM rats. FTIR spectra combined with Principal component analysis (PCA) showed, that treated DM rats by AV and HPO caused return chemical structure of blood and serum to this observed in control group. Higher similarity with control group for HPO rats was observed. HPO is better than AV in the alternative for healing on diabetic
wound. Thus, we have demonstrated that IR spectroscopy and multivariate data analysis and biochemical assays are consistent and correlative with each other.