Abstract | BACKGROUND: METHODS: We used recombinant TMPRSS2, reporter particles bearing the spike protein of SARS-CoV-2 or authentic SARS-CoV-2 to assess inhibition of TMPRSS2 and viral entry, respectively, by Camostat mesylate and its metabolite GBPA. FINDINGS: INTERPRETATION: Our results suggest that SARS-CoV-2 can use TMPRSS2 and closely related proteases for spread in the upper respiratory tract and that spread in the human lung can be blocked by Camostat mesylate and its metabolite GBPA. FUNDING: NIH, Damon Runyon Foundation, ACS, NYCT, DFG, EU, Berlin Mathematics center MATH+, BMBF, Lower Saxony, Lundbeck Foundation, Novo Nordisk Foundation.
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Authors | Markus Hoffmann, Heike Hofmann-Winkler, Joan C Smith, Nadine Krüger, Prerna Arora, Lambert K Sørensen, Ole S Søgaard, Jørgen Bo Hasselstrøm, Michael Winkler, Tim Hempel, Lluís Raich, Simon Olsson, Olga Danov, Danny Jonigk, Takashi Yamazoe, Katsura Yamatsuta, Hirotaka Mizuno, Stephan Ludwig, Frank Noé, Mads Kjolby, Armin Braun, Jason M Sheltzer, Stefan Pöhlmann |
Journal | EBioMedicine
(EBioMedicine)
Vol. 65
Pg. 103255
(Mar 2021)
ISSN: 2352-3964 [Electronic] Netherlands |
PMID | 33676899
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Antiviral Agents
- Esters
- Guanidines
- Membrane Proteins
- Protease Inhibitors
- camostat
- Serine Proteases
- Serine Endopeptidases
- TMPRSS11D protein, human
- TMPRSS13 protein, human
- TMPRSS2 protein, human
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Topics |
- Animals
- Antiviral Agents
(pharmacology)
- Cell Line
- Chlorocebus aethiops
- Cricetinae
- Esters
(pharmacology)
- Guanidines
(pharmacology)
- HEK293 Cells
- Humans
- Lung
(pathology, virology)
- Membrane Proteins
(biosynthesis)
- Molecular Dynamics Simulation
- Protease Inhibitors
(pharmacology)
- SARS-CoV-2
(drug effects)
- Serine Endopeptidases
(biosynthesis, metabolism)
- Serine Proteases
(biosynthesis)
- Vero Cells
- Virus Activation
(drug effects)
- Virus Internalization
(drug effects)
- COVID-19 Drug Treatment
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