Abstract |
Epilepsy is accompanied by abnormal neurotransmission, and microRNAs, as versatile players in the modulation of gene expression, are important in epilepsy pathology. Here, we found that miR-128 expression was elevated in the acute seizure phase and decreased during the recurrent seizure phase after status epilepticus in mice. Both SNAP-25 and SYT1 are regulated by miR-128 in vitro and in vivo. Overexpressing miR-128 in cultured neurons decreased neurotransmitter released by suppressing SNAP-25 and SYT1 expression. Anti-miR-128 injection before kainic acid (KA) injection increased the sensitivity of mice to KA-induced seizures, while overexpressing miR-128 at the latent and recurrent phases had a neuroprotective effect in KA-induced seizures. Our study shows for the first time that miR-128, a key regulator of neurotransmission, plays an important role in epilepsy pathology and that miR-128 might be a potential candidate molecular target for epilepsy therapy.
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Authors | Peng Wang, Yanchufei Zhang, Zihui Wang, Anyong Yang, Yuting Li, Qipeng Zhang |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 545
Pg. 195-202
(03 19 2021)
ISSN: 1090-2104 [Electronic] United States |
PMID | 33571908
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 Elsevier Inc. All rights reserved. |
Chemical References |
- MicroRNAs
- Mirn128 microRNA, mouse
- RNA, Messenger
- Snap25 protein, mouse
- Synaptosomal-Associated Protein 25
- Synaptotagmin I
- Syt1 protein, mouse
- Kainic Acid
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Topics |
- Animals
- Down-Regulation
- Epilepsy
(genetics, metabolism)
- Gene Knockdown Techniques
- Hippocampus
(drug effects, metabolism)
- Kainic Acid
(toxicity)
- Male
- Mice
- Mice, Inbred C57BL
- MicroRNAs
(antagonists & inhibitors, genetics, metabolism)
- RNA, Messenger
(genetics, metabolism)
- Seizures
(chemically induced, genetics, metabolism)
- Status Epilepticus
(genetics, metabolism)
- Synaptic Transmission
(genetics, physiology)
- Synaptosomal-Associated Protein 25
(genetics, metabolism)
- Synaptotagmin I
(genetics, metabolism)
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