Lipid Inhibitory Effect of (-)-loliolide Isolated from Sargassum horneri in 3T3-L1 Adipocytes: Inhibitory Mechanism of Adipose-Specific Proteins.

Abstract

Sargassum horneri (S. horneri) is a well-known brown seaweed widely distributed worldwide. Several biological activities of S. horneri have been reported. However, its effects on lipid metabolism and the underlying mechanisms remain elusive. In the present study, we examined the inhibitory effect of the active compound "(-)-loliolide ((6S,7aR)-6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydro-1-benzofuran-2(4H)-one (HTT))" from S. horneri extract on lipid accumulation in differentiated adipocytes. MTT assays demonstrated that (-)-loliolide is not toxic to 3T3-L1 adipocytes in a range of concentrations. (-)-loliolide significantly reduced intracellular lipid accumulation in the differentiated phase of 3T3-L1 adipocytes as shown by Oil Red O staining. Western blot analysis revealed that (-)-loliolide increased the expression of lipolytic protein phospho-hormone-sensitive lipase (p-HSL) and thermogenic protein peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1). Additionally, (-)-loliolide decreased expression of adipogenic and lipogenic proteins, including sterol regulatory element-binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer-binding protein-α (C/EBP-α), and fatty acid-binding protein 4 (FABP4) in 3T3-L1 adipocytes. These results indicate that (-)-loliolide from S. horneri could suppress lipid accumulation via regulation of antiadipogenic and prolipolytic mechanisms in 3T3-L1 cells. Considering the multifunctional effect of (-)-loliolide, it can be useful as a lipid-lowering agent in the management of patients who suffer from obesity.

• Authors: Hyo-Geun Lee, Hyun-Soo Kim, Jun-Geon Je, Jin Hwang, K K Asanka Sanjeewa, Dae-Sung Lee, Kyung-Mo Song, Yun-Sang Choi, Min-Cheol Kang, You-Jin Jeon
• Journal: Marine drugs (Mar Drugs) Vol. 19 Issue 2 (Feb 08 2021) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 33567534 (Publication Type: Journal Article)

Chemical References

• Benzofurans
• CCAAT-Enhancer-Binding Protein-alpha
• PPAR gamma
• Srebf1 protein, mouse
• Sterol Regulatory Element Binding Protein 1
• loliolide

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, metabolism)
• Animals
• Benzofurans (pharmacology)
• CCAAT-Enhancer-Binding Protein-alpha (antagonists & inhibitors)
• Lipid Metabolism (drug effects)
• Lipolysis (drug effects)
• Mice
• Obesity (drug therapy)
• PPAR gamma (antagonists & inhibitors)
• Sargassum (chemistry)
• Sterol Regulatory Element Binding Protein 1 (antagonists & inhibitors)
• Thermogenesis (drug effects)