Abstract |
Chondroitin sulfates are implicated in epidermal biology, but functional significance of chondroitin sulfates remains unclear. Here, we report that chondroitin 6-sulfate is important for the maintenance of epidermal homeostasis. Mice deficient in chondroitin 6-O-sulfotransferase-1 (C6st-1), which is involved in biosynthesis of chondroitin 6-sulfate, exhibited keratinocyte hyperproliferation and impaired skin permeability barrier function. Chondroitin 6-sulfate directly interacted with the EGF receptor and negatively controlled ligand-induced EGF receptor signaling. Normal function of hyperproliferative C6st-1-knockout mouse-derived keratinocytes was rescued by treatment with exogenous chondroitin 6-sulfate. Epidermal hyperplasia, induced using imiquimod, was more severe in C6st-1-knockout mice than in C6st-1 wild-type mice. Taken together, these findings indicate that chondroitin 6-sulfate represses keratinocyte proliferation in normal skin, and that the expression level of C6st-1 may be associated with susceptibility to psoriasis.
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Authors | Kazuyuki Kitazawa, Satomi Nadanaka, Kenji Kadomatsu, Hiroshi Kitagawa |
Journal | Communications biology
(Commun Biol)
Vol. 4
Issue 1
Pg. 114
(01 25 2021)
ISSN: 2399-3642 [Electronic] England |
PMID | 33495490
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chondroitin Sulfates
- Sulfotransferases
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Topics |
- Animals
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Chondroitin Sulfates
(metabolism, pharmacology)
- Epidermis
(drug effects, physiology)
- Genetic Predisposition to Disease
- HaCaT Cells
- Humans
- Keratinocytes
(drug effects, physiology)
- Mice
- Mice, Knockout
- Psoriasis
(genetics, metabolism, pathology)
- Skin
(cytology, drug effects)
- Sulfotransferases
(genetics, metabolism)
- Carbohydrate Sulfotransferases
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