Eugenol-piperine loaded polyhydroxy butyrate/polyethylene glycol nanocomposite-induced apoptosis and cell death in nasopharyngeal cancer (C666-1) cells through the inhibition of the PI3K/AKT/mTOR signaling pathway.

Abstract

Nasopharyngeal cancer is a malignancy developing from the nasopharynx epithelium due to smoking and nitrosamine-containing foods. Nasopharyngeal cancer is highly endemic to Southeast Asia. Eugenol and piperine have shown many anticancer activities on numerous cancer types, like colon, lung, liver, and breast cancer. In this study, we amalgamated eugenol and piperine loaded with a polyhydroxy butyrate/polyethylene glycol nanocomposite (Eu-Pi/PHB-PEG-NC) for better anticancer results against nasopharyngeal cancer (C666-1) cells. In the current study, nasopharyngeal cancer cell lines C666-1 were utilized to appraise the cytotoxic potential of Eug-Pip-PEG-NC on cell propagation, programmed cell death, and relocation. Eu-Pi/PHB-PEG-NC inhibits cellular proliferation on C666-1 cells in a dose-dependent manner, and when compared with 20 µg/ml, 15 µg/ml of loaded mixture evidently restrained the passage aptitude of C666-1 cells, this was attended with a downregulated expression of mitochondrial membrane potential. Treatment with 15 µg/ml Eu-Pi/PHB-PEG-NC suggestively amplified cell apoptosis in the C666-1 cells. Furthermore, its cleaved caspase-3, 8, and 9 and Bax gene expression was augmented and Bcl-2 gene expression was diminished after Eu-Pi/PHB-PEG-NC treatment. Additionally, our data established that the collective effect of Eu-Pi/PHB-PEG-NC loaded micelles inhibited the expansion of C666-1 cells augmented apoptosis connected with the intrusion of PI3K/Akt/mTOR signaling pathway.

• Authors: Xiaopeng Sun, Vishnu Priya Veeraraghavan, Krishna Mohan Surapaneni, Sardar Hussain, Maghimaa Mathanmohun, Sulaiman Ali Alharbi, Aref Ali Mohammed Aladresi, Arunachalam Chinnathambi
• Journal: Journal of biochemical and molecular toxicology (J Biochem Mol Toxicol) Vol. 35 Issue 4 Pg. e22700 (Apr 2021) ISSN: 1099-0461 [Electronic] United States
• PMID: 33421271 (Publication Type: Journal Article)
• Copyright: © 2021 Wiley Periodicals LLC.

Chemical References

• Alkaloids
• Benzodioxoles
• Drug Carriers
• Elafin
• PHB protein, human
• PI3 protein, human
• Piperidines
• Polyhydroxyalkanoates
• Polyunsaturated Alkamides
• Prohibitins
• Eugenol
• Polyethylene Glycols
• MTOR protein, human
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases
• piperine

Topics

• Alkaloids (chemistry, pharmacology)
• Apoptosis (drug effects)
• Benzodioxoles (chemistry, pharmacology)
• Cell Line, Tumor
• Drug Carriers (chemistry, pharmacology)
• Elafin (metabolism)
• Eugenol (chemistry, pharmacology)
• Humans
• Nanocomposites (chemistry, therapeutic use)
• Nasopharyngeal Neoplasms (drug therapy, metabolism, pathology)
• Piperidines (chemistry, pharmacology)
• Polyethylene Glycols (chemistry, pharmacology)
• Polyhydroxyalkanoates (chemistry, pharmacology)
• Polyunsaturated Alkamides (chemistry, pharmacology)
• Prohibitins
• Proto-Oncogene Proteins c-akt (metabolism)
• Signal Transduction (drug effects)
• TOR Serine-Threonine Kinases (metabolism)