Abstract | BACKGROUND: METHODS: We performed bioinformatics analysis, qRT-PCR, western blotting, immunohistochemistry, immunofluorescence, enzyme-linked immunosorbent, coimmunoprecipitation assays and a series of functional assays to investigate the roles of LEPR in hepatocellular carcinoma. RESULTS: We discovered that LEPR was highly expressed in liver cancer tissues, and the expression of LEPR in Hca-F cells was higher than that in Hca-P cells. Furthermore, LEPR promotes the proliferation, migration and invasion and inhibits the apoptosis of hepatocarcinoma lymphatic metastatic cells. Further studies indicated that LEPR interacts with ANXA7. Mechanistically, LEPR regulated ERK1/2 and JAK2/STAT3 expression via ANXA7 regulation. CONCLUSIONS: These findings unveiled a previously unappreciated role of LEPR in the regulation of lymphatic metastatic hepatocellular carcinoma, assigning ANXA7-LEPR as a promising therapeutic target for liver cancer treatments.
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Authors | He Huang, Jun Zhang, Fei Ling, Yuhong Huang, Min Yang, Yao Zhang, Yuanyi Wei, Qingqing Zhang, Honghai Wang, Lin Song, Ying Wu, Jiayu Yang, Jianwu Tang |
Journal | Cancer cell international
(Cancer Cell Int)
Vol. 21
Issue 1
Pg. 4
(Jan 04 2021)
ISSN: 1475-2867 [Print] England |
PMID | 33397392
(Publication Type: Journal Article)
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