Abstract | BACKGROUND: METHODS: In this study, SK-N-SH cells were used to establish an in vitro model to investigate the effects of BDMC on the Aβ1-42-induced neurotoxicity. SK-N-SH cells were pretreated with BDMC and with or without compound C and EX527 for 30 min after co-incubation with rotenone for 24 h. Subsequently, western blotting, cell viability assay and SOD and GSH activity measurement were performed. RESULTS: BDMC increased the cell survival, anti-OS ability, AMPK phosphorylation levels and SIRT1 in SK-N-SH cells treated with Aβ1-42. However, after treatment with compound C, an AMPK inhibitor, and EX527, an SIRT1inhibitor, the neuroprotective roles of BDMC on SK-N-SH cells treated with Aβ1-42 were inhibited. CONCLUSION: These results suggest that BDMC exerts a neuroprotective role on SK-N-SH cells in vitro via AMPK/ SIRT1 signaling, laying the foundation for the application of BDMC in the treatment of neurodegenerative diseases related to AMPK/ SIRT1 signaling.
|
Authors | Chenlin Xu, Zijian Xiao, Heng Wu, Guijuan Zhou, Duanqun He, Yunqian Chang, Yihui Li, Gang Wang, Ming Xie |
Journal | Translational neuroscience
(Transl Neurosci)
Vol. 11
Issue 1
Pg. 319-327
( 2020)
ISSN: 2081-3856 [Print] Germany |
PMID | 33335771
(Publication Type: Journal Article)
|
Copyright | © 2020 Chenlin Xu et al., published by De Gruyter. |