CDCP1-targeted nanoparticles encapsulating phase-shift perfluorohexan for molecular US imaging in vitro.

Abstract

BACKGROUND:

Molecular targeted contrast-enhanced ultrasound (CEUS) imaging is a potential imaging strategy to improve the diagnostic accuracy of conventional ultrasound (US) imaging. US contrast agents are usually micrometer-sized and non-target gas bubbles while nano-sized and targeted agents containing phase-shift materials absorb more attractions for their size and the liquid core and excellent molecular imaging effect.

METHODS:

PLGA12k-mPEG2k-NH2, DSPE-mPEG2k and perfluorohexan (PFH) were used to construct a new targeted ultrasound contrast agent with CUB domain-containing protein 1 (CDCP1) receptor for the detection and diagnosis of prostate cancer. The potential of tumor-targeted nanoparticles (CDCP1-targeted perfluorohexan-loaded phase-transitional nanoparticles, anti-CDCP1 NPs) as contrast agents for ultrasound (US) imaging was assessed in vitro. Moreover, studies on the cytotoxicity and the targeting ability of anti-CDCP1 NPs assisted by US were carried out.

RESULTS:

The results showed that anti-CDCP1 NPs had low cytotoxicity, and with the increasing of polymer concentration in anti-CDCP1 NPs, the CEUS imaging of agent gradually enhanced, and enhanced imaging associated with the length of observing time. Furthermore, it was testified that anti-CDCP1 assisted the agent to target cells expressing CDCP1, which demonstrated the active targeting of anti-CDCP1 NPs in vitro.

CONCLUSION:

All in all, the feasibility of using targeted anti-CDCP1 NPs to enhance ultrasound imaging has been demonstrated in vitro, which laid a solid foundation for molecular US imaging in vivo, and anti-CDCP1 NPs might have a great clinical application prospect.

• Authors: Meng Zhao, Yunkai Zhu, Yanhua Zhang, Xupeng Yang, Yourong Duan, Yaqing Chen, Ying Sun
• Journal: Clinical hemorheology and microcirculation (Clin Hemorheol Microcirc) Vol. 80 Issue 1 Pg. 25-35 ( 2022) ISSN: 1875-8622 [Electronic] Netherlands
• PMID: 33185589 (Publication Type: Journal Article)

Chemical References

• Contrast Media

Topics

• Cell Line, Tumor
• Contrast Media
• Humans
• Male
• Molecular Imaging
• Nanoparticles
• Ultrasonography