Long noncoding RNA U90926 is crucial for herpes simplex virus type 1 proliferation in murine retinal photoreceptor cells.

Abstract

Long non-coding RNAs (lncRNAs) play vital roles in the pathogenesis of infectious diseases, but the role of lncRNAs in herpes simplex virus 1 (HSV-1) infection remains unknown. Using RNA sequencing analysis, we explored lncRNAs that were highly expressed in murine retinal photoreceptor cell-derived 661W cells infected with HSV-1. U90926 RNA (522 nucleotides) was the most upregulated lncRNA detected post HSV-1 infection. The level of U90926 RNA was continuously increased post HSV-1 infection, reaching a 100-fold increase at 24 h. Cellular fractionation showed that U90926 RNA was located in the nucleus post HSV-1 infection. Downregulation of U90926 expression by RNA interference markedly suppressed HSV-1 DNA replication (80% reduction at 12 h post infection) and HSV-1 proliferation (93% reduction at 12 h post infection) in 661W cells. The survival rates of U90926-knockdown cells were significantly increased compared to those of control cells (81% and 21%, respectively; p < 0.0001). Thus, lncRNA U90926 is crucial for HSV-1 proliferation in retinal photoreceptor cells and consequently leads to host cell death by promoting HSV-1 proliferation.

• Authors: Shintaro Shirahama, Rena Onoguchi-Mizutani, Kentaro Kawata, Kenzui Taniue, Atsuko Miki, Akihisa Kato, Yasushi Kawaguchi, Rie Tanaka, Toshikatsu Kaburaki, Hidetoshi Kawashima, Yoshihiro Urade, Makoto Aihara, Nobuyoshi Akimitsu
• Journal: Scientific reports (Sci Rep) Vol. 10 Issue 1 Pg. 19406 (11 10 2020) ISSN: 2045-2322 [Electronic] England
• PMID: 33173149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Long Noncoding

Topics

• Animals
• Chlorocebus aethiops
• Herpesvirus 1, Human (genetics, pathogenicity)
• Mice, Inbred BALB C
• Mice, Knockout
• Photoreceptor Cells, Vertebrate (metabolism, virology)
• RNA, Long Noncoding (genetics, metabolism)
• Sequence Analysis, RNA
• Vero Cells
• Virus Replication (genetics, physiology)