β-Sitosterol attenuates liver injury in a rat model of chronic alcohol intake.

Abstract

Liver disease associated with long-term drinking is one of the leading causes of death. There is an urgent need for more effective drugs to reduce alcoholic liver damage. Yin Chen Hao, a traditional Chinese herbal medicine, is widely used for liver diseases. Here, we aimed to explore the protective effect of β-sitosterol (the active ingredient of Artemisia spp.) on alcoholic liver injuries. We treated the rats with alcohol and different dosages of β-sitosterol to detect the expression levels of liver function indicators in serum. The functions of β-sitosterol were evaluated based on variations in histology, liver function indicators and DNA oxidative damages. The underlying mechanism was investigated by measuring lipid peroxidation, the antioxidant, the expression of cytochrome P450 2E1 and the expression of apoptosis related genes. The results showed that β-sitosterol could improve liver histology and suppress biochemical indicators caused by alcohol in serum. In addition, β-sitosterol alleviates alcohol-induced oxidative stress, such as restoring erythrocyte membrane fluidity, reducing glutathione depletion, restoring antioxidant enzyme activity and reducing malondialdehyde overproduction. Furthermore, β-sitosterol downregulated the expression of apoptosis-related genes through the PI3K/Akt pathway. In conclusion, β-sitosterol has a protective effect on chronic alcoholism and has broad clinical application prospects in the treatment of alcohol-induced liver damage.

• Authors: Zhenjuan Chen, Ancheng Wu, Hongmei Jin, Fuhui Liu
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 43 Issue 11 Pg. 1197-1206 (Nov 2020) ISSN: 1976-3786 [Electronic] Korea (South)
• PMID: 33155166 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Apoptosis Regulatory Proteins
• Sitosterols
• gamma-sitosterol
• Phosphatidylinositol 3-Kinase
• Proto-Oncogene Proteins c-akt

Topics

• Alcohol Drinking
• Animals
• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (genetics, metabolism)
• DNA Damage
• Disease Models, Animal
• Lipid Peroxidation (drug effects)
• Liver (drug effects, metabolism, pathology)
• Liver Diseases, Alcoholic (etiology, metabolism, pathology, prevention & control)
• Male
• Oxidative Stress (drug effects)
• Phosphatidylinositol 3-Kinase (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• Rats, Wistar
• Sitosterols (pharmacology)