Abstract |
Stroke is one of the leading causes of death and disability in the world, which is associated with malfunction of reactive oxygen species and reactive nitrogen species (ROS/RNS) in cerebral microvessels. In vivo monitoring these species, such as ONOO-, with high selectivity in stroke process is of great significance for early diagnoses and therapies of the disease. Herein, by engineering an indoline-2,3-dione moiety as the recognizing domain, we proposed a novel fluorescence probe Rd-PN2 with highly specific response toward ONOO-, even in the coexistence of other ROS/RNS with high concentration. Rd-PN2 showed high sensitivity and reaction speed in response to ONOO- and exhibited satisfying performances in tracking the endogenously generated ONOO- in living cells and zebrafish. Accordingly, Rd-PN2 can furnish real-time and in vivo visualizing of ONOO- in cerebral microvessels of mice with ischemic and hemorrhagic strokes under two-photon microscopy. This work presented a precisely modulated fluorescence probe for real-time visualizing of ONOO- production in cerebral micovessels, which will also help to acquire more accurate information in the studies of ONOO- functions in the future.
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Authors | Jianhua Xiong, Weiwei Wang, Caixia Wang, Cheng Zhong, Renqiang Ruan, Zhiqiang Mao, Zhihong Liu |
Journal | ACS sensors
(ACS Sens)
Vol. 5
Issue 10
Pg. 3237-3245
(10 23 2020)
ISSN: 2379-3694 [Electronic] United States |
PMID | 33092345
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fluorescent Dyes
- Peroxynitrous Acid
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Topics |
- Animals
- Brain
- Fluorescent Dyes
- Mice
- Microvessels
- Peroxynitrous Acid
- Stroke
- Zebrafish
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