Lung cancer always ranks first in the number of
cancer deaths every year, accounting for 18.4% of total
cancer deaths in 2018.
Metastasis is the main cause of death in
lung cancer patients. The identification of bioactive components of
traditional Chinese medicine is very important for the development of novel
reagents against
non-small cell lung cancer (NSCLC).
Rosthorin A has originated from Rabdosia rosthornii (Diels) Hara which excerpts from 'Chinese
materia medica', and is known to have 'clear heat phlegm' properties in the folk. Little is known about the biological functions and mechanisms of
Rosthorin A in
cancer cells at present. The role of EMT in
metastasis of a
tumor cell is self-evident. Slug is an important EMT inducer, which is related to the development of
lung cancer. Cell growth, clone assay, cell migration, cell invasion, and
protein expression, and NSCLC transplanted
tumor growth were performed in A549, H1299, and H1975 cells.
Rosthorin A significantly inhibited the growth of NSCLC cells, it could prolong the survival of nude mice.
Rosthorin A inhibited the migration and invasion of A549, H1299, and H1975 cells.
Rosthorin A up-regulated
E-cadherin expression level and down-regulated the expression of β-
catenin,
N-cadherin,
vimentin, Slug, and Twist.
Rosthorin A could promote the expression of
E-cadherin and inhibit the development of EMT by downregulating Slug, to inhibit the development and
metastasis of NSCLC cells. In summary,
Rosthorin A could be used as a promising candidate for the treatment of NSCLC patients with recurrence and
metastasis.