Protective effects of nebivolol on ovarian ischemia-reperfusion injury in rat.

Abstract

AIM:

Ovarian torsion is a common gynecological emergency of reproductive ages, occurring at rates of 2.7-7.4%. This study aimed to evaluate the antioxidant effects of Nebivolol (NEB) and histopathological changes in experimental ischemic (I) and ischemic-reperfusion (I/R) injury in rat ovaries.

METHODS:

Forty-eight adult female rats were randomly separated into six groups as group 1 (control) receiving an oral saline solution for 3 days; group 2 (I) that underwent ischemia for 3 h with the application of atraumatic vascular clips; group 3 (I/R); group 4 (I + NEB) receiving 10 mg/kg NEB by oral gavage 30 min prior to the ischemia induction; group 5 (I/R + NEB) receiving 10 mg/kg NEB, and group 6 (control + NEB) receiving oral 10 mg/kg NEB for 3 days before ischemia induction followed by consequent reperfusion. Ovarian tissue damage was scored by histopathological analysis. Ovarian tissue malondialdehyde (MDA) and glutathione (GSH) levels were measured biochemically.

RESULTS:

The levels of MDA and tumor necrosis factor-alpha (TNF-α), and TUNEL assay positivity scores increased in the I and I/R groups. GSH levels decreased in all case groups (P < 0.05). The oral administration of NEB (10 mg/kg) to the I- and I/R-groups reduced the levels of MDA and TNF-α and TUNEL assay immunopositivity scores (P < 0.05). GSH levels increased in the treatment groups.

CONCLUSION:

The current experimental ovarian torsion study suggests a protective role for NEB against I and I/R injury in rat ovaries. NEB may be a novel agent for decreasing ovarian I/R injury.

• Authors: Sabri Colak, Beril Gurlek, Atilla Topcu, Levent Tumkaya, Tolga Mercantepe, Adnan Yilmaz
• Journal: The journal of obstetrics and gynaecology research (J Obstet Gynaecol Res) Vol. 46 Issue 11 Pg. 2407-2416 (Nov 2020) ISSN: 1447-0756 [Electronic] Australia
• PMID: 32961615 (Publication Type: Journal Article)
• Copyright: © 2020 Japan Society of Obstetrics and Gynecology.

Chemical References

• Antioxidants
• Nebivolol
• Malondialdehyde
• Glutathione

Topics

• Animals
• Antioxidants (pharmacology)
• Female
• Glutathione
• Humans
• Malondialdehyde
• Nebivolol (pharmacology)
• Ovarian Diseases (drug therapy, prevention & control)
• Ovary
• Rats
• Reperfusion Injury (drug therapy, prevention & control)