QiShenYiQi pill improves the reparative myocardial fibrosis by regulating autophagy.

Abstract

QiShenYiQi pill (QSYQ), a traditional Chinese medicine, is well known for improving the myocardial remodelling, but the dose-effect relationship of its intervention in the reparative myocardial fibrosis is still unclear. We investigated the effect of QSYQ on the reparative myocardial fibrosis in cardiac myosin-induced rats and explored its mechanism of action by regulating autophagy. The results indicated that QSYQ increased LVEF and LVFS, and decreased the LVEDD, LVESD, HMI, LVMI, myocardial inflammation histology score, and collagen volume fraction in a dose-dependent manner. In addition, QSYQ declined the number of autophagosomes, down-regulated the expression of myocardial Beclin-1 and LC3B, up-regulated the expression of myocardial p62 and increased the ratios of myocardial p-PI3K/PI3K, p-Akt/Akt and p-mTOR/mTOR. We provided evidence for that QSYQ could inhibit excessive myocardial autophagy by regulating the PI3K/Akt-mTOR pathway and can be a potential therapeutic approach in treating the cardiovascular diseases such as myocarditis and dilated cardiomyopathy.

• Authors: Shichao Lv, Peng Yuan, Jianping Dong, Chunmiao Lu, Meng Li, Fan Qu, Yaping Zhu, Zhuo Yuan, Junping Zhang
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 24 Issue 19 Pg. 11283-11293 (10 2020) ISSN: 1582-4934 [Electronic] England
• PMID: 32881330 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

Chemical References

• Autophagy-Related Proteins
• Beclin-1
• Drugs, Chinese Herbal
• LC3 protein, rat
• Microtubule-Associated Proteins
• RNA, Messenger
• Sequestosome-1 Protein
• Sqstm1 protein, rat
• qishen yiqi
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases

Topics

• Animals
• Autophagosomes (drug effects, metabolism, ultrastructure)
• Autophagy (drug effects)
• Autophagy-Related Proteins (metabolism)
• Beclin-1 (genetics, metabolism)
• Drugs, Chinese Herbal (pharmacology)
• Fibrosis
• Gene Expression Regulation (drug effects)
• Male
• Microtubule-Associated Proteins (genetics, metabolism)
• Myocardium (metabolism, pathology)
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats, Inbred Lew
• Sequestosome-1 Protein (genetics, metabolism)
• TOR Serine-Threonine Kinases (metabolism)