Objective: To investigate the in vitro antibacterial sensitivity of
levofloxacin,
tobramycin,
cefazolin sodium,
clindamycin and
fusidic acid to 67 strains of Staphylococci in ocular surface
infection. The purpose of this study is to provide reference for clinical selection of drugs. Methods: Experimental study. Sixth-seven strains of drug-resistant Staphylococci isolated from the Department of Microbiology, Henan Provincial Ophthalmic Hospital during January 2018 and May 2019 were collected. There were 67 strains of Staphylococci including 28 strains of drug-resistant Staphylococcus epidermidi, 17 strains of drug-resistant Staphylococcus aureus, 15 strains of drug-resistant Staphylococcus intermedius and a few other kinds of drug-resistant Staphylococci. The minimum inhibitory concentrations (minimum inhibitory concentration, MIC) of
levofloxacin,
tobramycin,
cefazolin sodium,
clindamycin and
fusidic acid in 67 strains of drug-resistant Staphylococci were determined by microliquid-based method. The sensitivity was determined according to the American CLSI-M100 standard. The statistical analysis of the data was carried out by using two-dimensional test and Fisher accurate test. Results: Fourteen strains of
fusidic acid were sensitive to 17 strains of MRS-
Meca-resistant Staphylococcus epidermidis, the difference between fusidic and
levofloxacin is statistically significant; 14 strains of
cefazolin sodium and 11 strains of
fusidic acid were sensitive to 14 strains of β-Lac
enzyme-producing Staphylococcus aureus, and there were significant differences between the two drugs and
levofloxacin; 6 strains of
cefazolin sodium and 5 strains of
fusidic acid were sensitive to 10 strains of MRS-
Meca-resistant Staphylococcus intermedius, as compared to
levofloxacin, there were significant differences between
cefazolin sodium and
levofloxacin (P=0.011,0.033).
Cefazolin sodium was sensitive to 5 strains of MRS-
Meca-positive other drug-resistant Staphylococci, which was significantly different from
levofloxacin (P=0.048); 54 and 48 strains of
cefazolin sodium and
fusidic acid were sensitive to 67 strains of drug-resistant Staphylococci, and the sensitive rates were 80.1% and 71.6%, respectively, which were significantly higher than those of
levofloxacin,
tobramycin and
clindamycin. There were significant statistical differences between drug sensitivity (χ²=18.377,9.940;P=0.000,0.003). Conclusions: The sensitivity of
cefazolin sodium and
fusidic acid to 67 strains of drug-resistant Staphylococci is better than that of
levofloxacin,
tobramycin and
clindamycin, these findings may provide guidance for the clinical treatment of drug-resistant Staphylococci in
ocular infection. (Chin J Ophthalmol, 2020, 56: 621-625).