Circular RNA has circ 0001591 promoted cell proliferation and metastasis of human melanoma via ROCK1/PI3K/AKT by targeting miR-431-5p.

Abstract

Melanoma a common skin tumor induced by excessive hyperplasia of abnormal melanocyte. Circular RNAs (circRNAs) play critical roles in various diseases and presented as the prognostic markers of melanoma. The present study was designed to confirm the effect of circ 0001591 on human melanoma cell growth and to elucidate its mechanism. Patient with melanoma was obtained from Shaanxi Provincial People's Hospital. Cell viability of A2058 cell was detected by MTT assay. The expression of circ 0001591 in serum of patients with melanoma was increased. Up-regulation of circ 0001591 promoted cell growth and cell invasion, and reduced apoptotic rate of melanoma. Down-regulation of circ 0001591 reduced cell growth and cell invasion, and promoted apoptotic rate of melanoma.Up-regulation of circ 0001591 induced PI3 K and p-Akt protein expressions in melanoma through induction of ROCK1 by suppression of miR-431-5p. Over-expression of circ 0001591 suppressed PI3 K and p-Akt protein expressions via suppression of ROCK1 in melanoma by induction of miR-431-5p. MiR-431-5p reduced the effects of circ 0001591 down-regulation on cell growth of melanoma through PI3K/AKT signal pathway. ROCK1 reduced the effects of circ 0001591 on cell growth of melanoma through PI3K/AKT signal pathway. Our findings demonstrated that circ 0001591 inhibits the progression of human melanoma through ROCK1/PI3K/AKT signal pathway by targeting ROCK1 by miR-431-5p.

• Authors: Dong Yin, Guo Wei, Fan Yang, Xiaoyan Sun
• Journal: Human & experimental toxicology (Hum Exp Toxicol) Vol. 40 Issue 2 Pg. 310-324 (Feb 2021) ISSN: 1477-0903 [Electronic] England
• PMID: 32830578 (Publication Type: Journal Article)

Chemical References

• MIRN431 microRNA, human
• MicroRNAs
• RNA, Circular
• Proto-Oncogene Proteins c-akt
• ROCK1 protein, human
• rho-Associated Kinases

Topics

• Apoptosis
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Humans
• Melanoma (blood, genetics, metabolism, pathology)
• MicroRNAs
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• RNA, Circular
• Signal Transduction
• Skin Neoplasms (blood, genetics, metabolism, pathology)
• rho-Associated Kinases (metabolism)