Abstract | BACKGROUND: MATERIALS AND METHODS: Immunohistochemical analysis and qRT-PCR were used to detect the expression of PPAT in thyroid cancer samples. Both gain-of-function and loss-of-function models were constructed in thyroid cancer cell lines and the biological functions of PPAT on cellular phenotypes were studied using CCK-8 assay and transwell assay in vitro, respectively. Then, Western blot was used to evaluate the change of PKM2 and downstream signal pathways after PPAT was overexpressed or knocked down. RESULTS: Immunohistochemical analysis showed increased expression of PPAT in thyroid cancer tissues, and it was associated with unfavorable pathological characteristics. Knockdown and overexpression assays suggested that altering PPAT expression modulated cell proliferation, migration, and invasion. In terms of mechanism, PPAT could positively regulate the expression of PKM2 and activate ERK and STAT3 signaling pathways. CONCLUSION: PPAT plays crucial roles in regulating proliferation, migration, and invasion of thyroid cancer cells via activating PKM2, ERK, and STAT3.
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Authors | Bing Liu, Meiyue Song, Huadong Qin, Bin Zhang, Yao Liu, Yu Sun, Yanfei Ma, Tiefeng Shi |
Journal | OncoTargets and therapy
(Onco Targets Ther)
Vol. 13
Pg. 7629-7639
( 2020)
ISSN: 1178-6930 [Print] New Zealand |
PMID | 32801776
(Publication Type: Journal Article)
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Copyright | © 2020 Liu et al. |