The invasive proteome of glioblastoma revealed by laser-capture microdissection.

Abstract

BACKGROUND:

Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery.

METHODS:

Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.

RESULTS:

Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were subsequently validated in tumors and by functional assays.

CONCLUSIONS:

In summary, our results identify new networks and molecules that may play an important role in glioblastoma development and may constitute potential novel therapeutic targets.

• Authors: Thomas Daubon, Joris Guyon, Anne-Aurélie Raymond, Benjamin Dartigues, Justine Rudewicz, Zakaria Ezzoukhry, Jean-William Dupuy, John M J Herbert, Frédéric Saltel, Rolf Bjerkvig, Macha Nikolski, Andreas Bikfalvi
• Journal: Neuro-oncology advances (Neurooncol Adv) 2019 May-Dec Vol. 1 Issue 1 Pg. vdz029 ISSN: 2632-2498 [Electronic] England
• PMID: 32642662 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2019. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.