Titanium particles damage osteocytes and inhibit osteoblast differentiation.

Abstract

PURPOSES:

to study the effect of titanium particles on MLO-Y4 and the effects of osteocytes alterations on osteoblasts.

METHODS:

cultured MLO-Y4 osteocytes were exposed to different concentrations of titanium (Ti) particles, cell viability was measured using the Cell Counting Kit-8 (CCK-8) assay, apoptosis of MLO-Y4 cells was evaluated by flow cytometry, Real-time PCR quantification of mRNA expression of SOST, at the same time with Western Blot detection sclerosteosis protein expression levels.MC3T3-E1 cells culture with MLO-Y4 cells exposed to different concentrations of titanium (Ti) particles in vitro, in order to detection of osteoblast osteogenetic activity.

RESULTS:

Our results showed that Ti particles inhibited cell viability of MLO-Y4 osteocytes in a dose-dependent manner. Incubation with Ti particles caused apoptosis of MLO-Y4cells.Treatment with Ti particles significantly increased expression of the osteocytic marker SOST/sclerostin. Furthermore, treatment of MLO-Y4 cells with Ti particles produced a dose-dependent decrease in ALP activity and decreased mineralization of MC3T3-E1 cells through direct cell-cell contact.

CONCLUSIONS:

Titanium particles damage osteocytes and inhibit osteoblast differentiation.

• Authors: Li Chen, Ziyue Wang, Wei Xu, Qirong Dong
• Journal: Journal of experimental orthopaedics (J Exp Orthop) Vol. 7 Issue 1 Pg. 47 (Jul 04 2020) ISSN: 2197-1153 [Print] Germany
• PMID: 32623526 (Publication Type: Journal Article, Retracted Publication)