Serum interleukin-8 as a potential diagnostic biomarker in esophageal squamous cell carcinoma.

Abstract

BACKGROUND:

Esophageal squamous cell carcinoma (ESCC) has poor prognosis mainly due to the difficulty of making early diagnosis. Therefore, novel biomarkers are critically needed.

OBJECTIVE:

We aimed to investigate the diagnostic value of serum interleukin-8 (IL-8) in ESCC.

METHODS:

Data mining of TCGA was used to analyze expression level of IL-8 mRNA in esophageal carcinoma. Serum levels of IL-8 were measured in 103 ESCC patients and 86 normal controls by ELISA. Receiver operating characteristic (ROC) curve was used to evaluate its diagnostic accuracy.

RESULTS:

IL-8 mRNA expression level and serum IL-8 concentration were both statistically higher in patients than normal controls (P< 0.001). ROC curve demonstrated that the optimum diagnostic cut-off for serum IL-8 was 80.082 pg/mL, providing an area under the curve (AUC) of 0.694 (95% CI: 0.620-0.768), with specificity of 86.0% and sensitivity of 42.7%. The AUC for early-stage ESCC was 0.618 (95% CI: 0.499-0.737), with sensitivity of 35.3% and specificity of 86.0%. Kaplan-Meier analysis and the log-rank test indicated that IL-8 may not be a prognostic predictor for ESCC.

CONCLUSIONS:

Serum IL-8 was highly expressed in ESCC patients and may be a potential marker for early diagnosis of ESCC.

• Authors: Zeting Huang, Bin Zhou, Zheng Li, Cantong Liu, Chunwen Zheng, Ruijie Zeng, Chaoqun Hong, Liyan Xu, Enmin Li, Yuhui Peng, Yiwei Xu
• Journal: Cancer biomarkers : section A of Disease markers (Cancer Biomark) Vol. 29 Issue 1 Pg. 139-149 ( 2020) ISSN: 1875-8592 [Electronic] Netherlands
• PMID: 32623391 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Interleukin-8
• RNA, Messenger

Topics

• Adult
• Aged
• Biomarkers, Tumor (blood, genetics)
• Esophageal Squamous Cell Carcinoma (blood, genetics, pathology)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Interleukin-8 (blood, genetics)
• Kaplan-Meier Estimate
• Male
• Middle Aged
• Prognosis
• RNA, Messenger (blood)