Analysis of TSH receptors and microsomal antigen in different human thyroid tissue specimens.

Abstract

Affinity labelling with a 125I-labelled photoactive derivative of TSH (HSAB-TSH) was used to analyse TSH receptor size in the following specimens of human thyroid tissue: (1) cold nodules; (2) autonomous nodules; (3) papillary carcinoma; (4) medullary carcinoma; (5) metastasis of papillary carcinoma to lymph node; (6) anaplastic carcinoma, and (7) Graves' thyroid. In addition, a sample of histologically normal thyroid tissue surrounding specimens 1-4 was analysed in each case. Thyroid microsomes were also prepared from the tissue samples, solubilized using 1% deoxycholate and labelled with 125I. The preparations were immunoprecipitated using microsomal autoantibodies and protein A and analysed by SDS-PAGE and autoradiography. These studies indicated that no differences in the characteristics of the TSH receptor or of microsomal antigen were observed in the tissue samples 1-3 and 7. Neither protein was detected in tissue specimens 4-6.

• Authors: J Furmaniak, S Davenport, Y Nakajima, U Kaiser, H W Muller-Gartner, F W Buckmann, C Pegg, B Rees Smith
• Journal: Clinical endocrinology (Clin Endocrinol (Oxf)) Vol. 28 Issue 6 Pg. 589-600 (Jun 1988) ISSN: 0300-0664 [Print] England
• PMID: 3254258 (Publication Type: Journal Article)

Chemical References

• Autoantigens
• Receptors, Thyrotropin

Topics

• Autoantigens (analysis)
• Autoradiography
• Electrophoresis, Polyacrylamide Gel
• Humans
• Microsomes (immunology)
• Receptors, Thyrotropin (analysis)
• Thyroid Diseases (immunology)
• Thyroid Gland (immunology)