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Proteomic Analysis of Metastasis-Specific Biomarkers in Pancreatic Cancer: Galectin-1 Plays an Important Metastatic Role in Pancreatic Cancer.

Abstract
Cancer metastasis is the major cause of death in pancreatic cancer. We have established a pair of pancreatic ductal adenocarcinoma cell line, PANC1 and invasive PANC1-I5, as a model system toinvestigate the metastatic mechanism as well as potential therapeutic targets in pancreatic cancer. We used proteomic analysis based on two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to examine the global protein expression alterations between PANC1 and PANC1-I5. Proteomic study revealed that 88 proteins are differentially expressed between PANC1-I5 and PANC1 cells, and further functional evaluations through protein expression validation, gene knockout, migration and invasion analysis revealed that galectin-1 is one of the potential players in modulating pancreatic cancer metastasis. To conclude, we have identified numerous proteins might be associated with pancreatic cancer invasiveness in the pancreatic cancer model.
AuthorsChun-Liang Tung, Meng-Wei Lin, Ren-Yu Hu, Yi-Chun Chien, En-Chi Liao, Li-Hsun Lin, Ting-Wen Chung, Yu-Shan Wei, Yi-Ting Tsai, Hsin-Yi Chen, Hsiu-Chuan Chou, Wen-Hung Kuo, Mei-Lan Ko, Shing-Jyh Chang, Ying-Ray Lee, Hong-Lin Chan
JournalJournal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 186 Pg. 113300 (Jul 15 2020) ISSN: 1873-264X [Electronic] England
PMID32413824 (Publication Type: Journal Article)
CopyrightCopyright © 2020 Elsevier B.V. All rights reserved.
Chemical References
  • Biomarkers, Tumor
  • Galectin 1
  • LGALS1 protein, human
Topics
  • Adenocarcinoma (genetics, metabolism, pathology)
  • Biomarkers, Tumor (metabolism)
  • Carcinoma, Pancreatic Ductal (genetics, metabolism, pathology)
  • Cell Line, Tumor
  • Cell Movement
  • Galectin 1 (metabolism)
  • Gene Expression Regulation, Neoplastic
  • Gene Knockout Techniques
  • Humans
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Pancreatic Neoplasms (genetics, metabolism, pathology)
  • Proteomics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Two-Dimensional Difference Gel Electrophoresis

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