Abstract |
Cancer metastasis is the major cause of death in pancreatic cancer. We have established a pair of pancreatic ductal adenocarcinoma cell line, PANC1 and invasive PANC1-I5, as a model system toinvestigate the metastatic mechanism as well as potential therapeutic targets in pancreatic cancer. We used proteomic analysis based on two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to examine the global protein expression alterations between PANC1 and PANC1-I5. Proteomic study revealed that 88 proteins are differentially expressed between PANC1-I5 and PANC1 cells, and further functional evaluations through protein expression validation, gene knockout, migration and invasion analysis revealed that galectin-1 is one of the potential players in modulating pancreatic cancer metastasis. To conclude, we have identified numerous proteins might be associated with pancreatic cancer invasiveness in the pancreatic cancer model.
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Authors | Chun-Liang Tung, Meng-Wei Lin, Ren-Yu Hu, Yi-Chun Chien, En-Chi Liao, Li-Hsun Lin, Ting-Wen Chung, Yu-Shan Wei, Yi-Ting Tsai, Hsin-Yi Chen, Hsiu-Chuan Chou, Wen-Hung Kuo, Mei-Lan Ko, Shing-Jyh Chang, Ying-Ray Lee, Hong-Lin Chan |
Journal | Journal of pharmaceutical and biomedical analysis
(J Pharm Biomed Anal)
Vol. 186
Pg. 113300
(Jul 15 2020)
ISSN: 1873-264X [Electronic] England |
PMID | 32413824
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers, Tumor
- Galectin 1
- LGALS1 protein, human
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Topics |
- Adenocarcinoma
(genetics, metabolism, pathology)
- Biomarkers, Tumor
(metabolism)
- Carcinoma, Pancreatic Ductal
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
- Galectin 1
(metabolism)
- Gene Expression Regulation, Neoplastic
- Gene Knockout Techniques
- Humans
- Neoplasm Invasiveness
- Neoplasm Metastasis
- Pancreatic Neoplasms
(genetics, metabolism, pathology)
- Proteomics
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Two-Dimensional Difference Gel Electrophoresis
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