Abstract |
AIM: To analyze the expression patterns of various AP forms in cells originated from CML patients in blast crisis and to modify their expression by vitamin E. MATERIALS AND METHODS: RESULTS: We have observed the aberrant expression of mRNA intestinal alkaline phosphatase in CML cells that upon sequencing demonstrated the significant alignment with PLAP sequence while no gene homology with tissue placental alkaline phosphatase (PAP) was revealed. Vitamin E decreases mRNA PLAP expression and increases mRNA TNAP expression. Moreover, along with down-regulation of aberrant PLAP and up-regulation of TNAP, vitamin E increases C/EBPα mRNA expression. CONCLUSION: The loss of TNAP in CML may contribute to pathogenesis of this disease. PLAP may be considered as a putative target in differentiation therapies in myeloid neoplasms. Our findings suggest the potential role of vitamin E as the inducer of differentiation potential of leukemic cells in CML.
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Authors | L P Shvachko, M P Zavelevich, D F Gluzman, G D Telegeev |
Journal | Experimental oncology
(Exp Oncol)
Vol. 42
Issue 1
Pg. 31-34
(03 2020)
ISSN: 1812-9269 [Print] Ukraine |
PMID | 32231189
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Isoenzymes
- germ-cell AP isoenzyme
- Vitamin E
- Alkaline Phosphatase
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Topics |
- Alkaline Phosphatase
(genetics)
- Biomarkers, Tumor
(genetics)
- Blast Crisis
(enzymology, genetics, pathology)
- Cell Line, Tumor
- Down-Regulation
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Isoenzymes
(genetics)
- K562 Cells
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive
(enzymology, genetics, pathology)
- Up-Regulation
- Vitamin E
(pharmacology)
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