LOC101060264 Silencing Suppresses Invasion and Metastasis of Human Colon Cancer.

Abstract

BACKGROUND We explored the regulatory effects of long noncoding RNA (lncRNA) LOC101060264 silencing mediated by shRNA on invasion and metastasis of human colon cancer. MATERIAL AND METHODS Initially, 2 shRNA plasmids for LOC101060264 silencing - shRNA1 and shRNA2 - were introduced into LoVo cells. Following transfection, the expressions of LOC101060264, E-cadherin, and vimentin were determined. Next, to explore the regulatory effects of LOC101060264 silencing on cell growth, cell cycle, invasion, and migration abilities of LoVo cells, we performed MTT, flow cytometry, Transwell assay, and scratch assay, respectively. Furthermore, in nude mice with xenografted tumors, the tumor volume and weight were measured, and the expressions of PCNA, E-cadherin, vimentin, and MMP-9 in tumor tissues were determined by immunohistochemistry. RESULTS The level of E-cadherin increased and the level of vimentin decreased after LOC101060264 silencing mediated by shRNA1 and shRNA2 in LoVo cells. Silencing LOC101060264 repressed the migration, invasion, and proliferation of LoVo cells in vitro and inhibited tumor growth in nude mice in vivo. We also studied the expression of these proteins and found reduced expression of PCNA, vimentin, and MMP-9 protein, and found enhanced expression of E-cadherin protein. Moreover, the inhibitory effect of shRNA2 on the above cell behaviors was stronger than that of shRNA1. CONCLUSIONS In summary, LOC101060264 silencing decreased LoVo cell invasiveness via suppressing ETM and attenuated tumor metastasis, which provides a novel therapeutic target for patients with colon cancer.

• Authors: Weihua Yu, Yunxia Wang, Lan Liu, Shuai Li, Kongxi Zhu
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 26 Pg. e920270 (Feb 20 2020) ISSN: 1643-3750 [Electronic] United States
• PMID: 32077446 (Publication Type: Journal Article)

Chemical References

• Cadherins
• Proliferating Cell Nuclear Antigen
• RNA, Long Noncoding
• Vimentin
• Matrix Metalloproteinase 9

Topics

• Animals
• Cadherins (genetics, metabolism)
• Cell Cycle (genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Cell Survival (genetics)
• Colonic Neoplasms (genetics, pathology)
• Down-Regulation (genetics)
• Gene Expression Regulation, Neoplastic
• Gene Silencing
• Humans
• Male
• Matrix Metalloproteinase 9 (metabolism)
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Invasiveness
• Neoplasm Metastasis
• Proliferating Cell Nuclear Antigen (metabolism)
• RNA, Long Noncoding (genetics, metabolism)
• Tumor Burden (genetics)
• Vimentin (genetics, metabolism)