Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology.

Abstract

BACKGROUND:

As an effective prescription for gastric cancer (GC), Compound Kushen Injection (CKI) has been widely used even though few molecular mechanism analyses have been carried out.

METHODS:

In this study, we identified 16 active ingredients and 60 GC target proteins. Then, we established a compound-predicted target network and a GC target protein-protein interaction (PPI) network by Cytoscape 3.5.1 and systematically analyzed the potential targets of CKI for the treatment of GC. Finally, molecular docking was applied to verify the key targets. In addition, we analyzed the mechanism of action of the predicted targets by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses.

RESULTS:

The results showed that the potential targets, including CCND1, PIK3CA, AKT1, MAPK1, ERBB2, and MMP2, are the therapeutic targets of CKI for the treatment of GC. Functional enrichment analysis indicated that CKI has a therapeutic effect on GC by synergistically regulating some biological pathways, such as the cell cycle, pathways in cancer, the PI3K-AKT signaling pathway, the mTOR signaling pathway, and the FoxO signaling pathway. Moreover, molecular docking simulation indicated that the compounds had good binding activity to PIK3CA, AKT1, MAPK1, ERBB2, and MMP2 in vivo.

CONCLUSION:

This research partially highlighted the molecular mechanism of CKI for the treatment of GC, which has great potential in the identification of the effective compounds in CKI and biomarkers to treat GC.

• Authors: Wei Zhou, Jiarui Wu, Yingli Zhu, Ziqi Meng, Xinkui Liu, Shuyu Liu, Mengwei Ni, Shanshan Jia, Jingyuan Zhang, Siyu Guo
• Journal: BMC complementary medicine and therapies (BMC Complement Med Ther) Vol. 20 Issue 1 Pg. 6 (Jan 15 2020) ISSN: 2662-7671 [Electronic] England
• PMID: 32020871 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Drugs, Chinese Herbal
• kushen

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Humans
• Injections
• Molecular Docking Simulation
• Protein Interaction Maps
• Signal Transduction
• Stomach Neoplasms (drug therapy)