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Impact of endogenous stress on albumin structure in systemic lupus erythematosus (SLE) patients.

Abstract
Systemic lupus erythematosus (SLE) is an inflammatory, autoimmune disorder of unknown etiology. The inflammatory stress in SLE patients may modify macromolecules and produce structural/functional abnormalities. The present study is aimed at examining the consequences of stresses on the structure of albumin in SLE patients. Albumin was isolated from the sera of SLE/healthy subjects. Multiple physicochemical techniques were used to elucidate, structure of albumin. Advanced glycation end products in SLE patients' albumin were identified by the AGE specific fluorescence. Quenching of tryptophan, tyrosine fluorescence and surface protein hydrophobicity was observed in SLE patients' albumin. Protein-bound carbonyls were elevated while free thiol, lysine, arginine, and alpha helicity was found to be decreased in SLE albumin. Furthermore, changes in the secondary structure of SLE albumin were observed as shift in the position of amide I/II bands. Functionality of SLE albumin was also compromised as its cobalt-binding ability was substantially declined. Adduction of moieties was detected by dynamic light scattering (DLS) and confirmed by matrix assisted laser desorption/ionization. DLS, thioflavin T and transmission electron microscopy results confirmed aggregates in SLE patients' albumin. This study may be helpful in understanding the role of modified albumin in the cofounding pathologies associated with SLE.
AuthorsZarina Arif, Akhlas Tarannum, Mir Yasir Arfat, Binish Arif, Sana Shahab, Maryam Arif, Km Nelofar, Asim Badar, Shireen Naaz Islam, Asif Zaman, Shafeeque Ahmad, Mohammad Arif Iqubal, Akankcha Gupta, Amita Aggarwal, Khursheed Alam
JournalInternational journal of biological macromolecules (Int J Biol Macromol) Vol. 151 Pg. 891-900 (May 15 2020) ISSN: 1879-0003 [Electronic] Netherlands
PMID32014478 (Publication Type: Journal Article)
CopyrightCopyright © 2020 Elsevier B.V. All rights reserved.
Chemical References
  • Albumins
  • Protein Aggregates
Topics
  • Adolescent
  • Adult
  • Aged
  • Albumins (chemistry)
  • Female
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Lupus Erythematosus, Systemic
  • Male
  • Middle Aged
  • Oxidation-Reduction
  • Oxidative Stress
  • Protein Aggregates
  • Protein Conformation
  • Spectrum Analysis
  • Stress, Physiological
  • Young Adult

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