Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer.

Abstract

As the area of small molecules interacting with RNA advances, general routes to provide bioactive compounds are needed as ligands can bind RNA avidly to sites that will not affect function. Small-molecule targeted RNA degradation will thus provide a general route to affect RNA biology. A non-oligonucleotide-containing compound was designed from sequence to target the precursor to oncogenic microRNA-21 (pre-miR-21) for enzymatic destruction with selectivity that can exceed that for protein-targeted medicines. The compound specifically binds the target and contains a heterocycle that recruits and activates a ribonuclease to pre-miR-21 to substoichiometrically effect its cleavage and subsequently impede metastasis of breast cancer to lung in a mouse model. Transcriptomic and proteomic analyses demonstrate that the compound is potent and selective, specifically modulating oncogenic pathways. Thus, small molecules can be designed from sequence to have all of the functional repertoire of oligonucleotides, including inducing enzymatic degradation, and to selectively and potently modulate RNA function in vivo.

• Authors: Matthew G Costales, Haruo Aikawa, Yue Li, Jessica L Childs-Disney, Daniel Abegg, Dominic G Hoch, Sai Pradeep Velagapudi, Yoshio Nakai, Tanya Khan, Kye Won Wang, Ilyas Yildirim, Alexander Adibekian, Eric T Wang, Matthew D Disney
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 117 Issue 5 Pg. 2406-2411 (02 04 2020) ISSN: 1091-6490 [Electronic] United States
• PMID: 31964809 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 the Author(s). Published by PNAS.

Chemical References

• MIRN21 microRNA, mouse
• MicroRNAs
• Oligonucleotides
• Small Molecule Libraries
• Ribonucleases

Topics

• Animals
• Breast Neoplasms (drug therapy, pathology)
• Cell Line, Tumor
• Disease Models, Animal
• Drug Design
• Female
• Humans
• Mice
• MicroRNAs (chemistry, metabolism)
• Molecular Structure
• Neoplasm Metastasis
• Oligonucleotides (chemistry, metabolism)
• Ribonucleases (chemistry, metabolism)
• Small Molecule Libraries (chemistry, metabolism, therapeutic use)