Silencing NOB1 Can Affect Cell Proliferation and Apoptosis Via the C-Jun N-Terminal Kinase Pathway in Colorectal Cancer.

Abstract

OBJECTIVES:

To investigate the bio-functions and the molecular mechanisms of NIN1/proteasome 26S subunit non-ATPase 8 binding protein 1 homolog (NOB1) in colorectal cancer cells.

METHODS:

NOB1 expression was silenced using si-RNA in SW480 and LoVo cells. The transfection efficiency was measured by western blotting and RT-qPCR. Subsequently, the proliferation of SW480 and LoVo cells was determined using both MTT assay and colony-formation assay. Apoptosis and cell cycle analysis were determined using flow cytometry.

RESULTS:

Compared with the normal control (NC) and scramble cells, si-NOB1 could significantly attenuate the proliferation, colony-formation ability and cell percentage of S stage (p < 0.05). Additionally, at the phosphorylation level, si-NOB1 could notably increase the expression of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38.

CONCLUSIONS:

Inhibition of NOB1 expression suppressed the proliferation, and promoted the apoptosis through regulation of the JNK signaling pathway.

• Authors: Zhong Ren, Liqing Yao, Jingzheng Liu, Zhipeng Qi, Jian Li
• Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research (J Invest Surg) Vol. 34 Issue 8 Pg. 819-825 (Aug 2021) ISSN: 1521-0553 [Electronic] United States
• PMID: 31906747 (Publication Type: Journal Article)

Chemical References

• NOB1 protein, human
• Nuclear Proteins
• RNA-Binding Proteins
• JNK Mitogen-Activated Protein Kinases

Topics

• Apoptosis
• Cell Line, Tumor
• Cell Proliferation
• Colorectal Neoplasms (genetics)
• Humans
• JNK Mitogen-Activated Protein Kinases
• Nuclear Proteins (metabolism)
• RNA-Binding Proteins (genetics)