Abstract | OBJECTIVE:
Breast cancer (BC) is a common malignancy all over the world. However, the detailed mechanism underlying BC progression remains incompletely understood. MicroRNAs ( miRNAs) have been observed to play crucial roles in tumorigenesis. The present study aimed to determine the expression and function of miR-296 in BC. PATIENTS AND METHODS: MiR-296 expressions in BC tissue samples and cell lines were examined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). After that, we performed functional assays, including MTT (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays and transwell assays, to show the functions of miR-296 in BC cell proliferation, invasion and migration. Immunological histological chemistry (IHC) assays were carried out to detect the expression levels of fibroblast growth factor receptor 1 (FGFR1) in BC tissue samples. Western blot was used to explore potential mechanisms of miR-296 in regulating BC progression. A Luciferase reporter assay was carried out to confirm the target gene of miR-296. RESULTS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) results demonstrated a significant decrease of miR-296 expressions in BC when compared to the corresponding normal controls. In addition, the decreased miR-296 was correlated with the malignant phenotypes and poorer prognosis of BC patients. The functional assays indicated that miR-296 restoration could repress the proliferation, invasion and migration abilities of BC cells. Moreover, the results of the current study revealed that miR-296 exerted the repressive functions in BC cells via regulating FGFR1, the Wnt/β- catenin signaling pathway and EMT. Additionally, miR-296 up-regulation could inhibit in vivo BC cell growth. CONCLUSIONS: All these findings indicated that miR-296 exerted anti-BC functions, providing novel therapeutic strategies in BC treatment.
|
Authors | W-M Sun, W Tao, J-C Li, D-M Zhu, Y Miao |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 23
Issue 23
Pg. 10422-10432
(Dec 2019)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 31841196
(Publication Type: Journal Article)
|
Chemical References |
- MIRN296 microRNA, human
- MicroRNAs
- FGFR1 protein, human
- Receptor, Fibroblast Growth Factor, Type 1
|
Topics |
- Animals
- Breast
(pathology, surgery)
- Breast Neoplasms
(genetics, mortality, pathology, surgery)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
(genetics)
- Disease Progression
- Down-Regulation
- Female
- Gene Expression Regulation, Neoplastic
- Genes, Tumor Suppressor
- Humans
- Kaplan-Meier Estimate
- Mastectomy
- Mice
- MicroRNAs
(genetics, metabolism)
- Middle Aged
- Neoplasm Invasiveness
(genetics)
- Prognosis
- Receptor, Fibroblast Growth Factor, Type 1
(genetics)
- Wnt Signaling Pathway
(genetics)
- Xenograft Model Antitumor Assays
|