MiR-501-3p functions as a tumor suppressor in non-small cell lung cancer by downregulating RAP1A.

Abstract

MicroRNA-501-3p (miR-501-3p) has been reported to play tumor-suppressive roles in different cancers; however, its expression pattern and biological function in non-small cell lung cancer (NSCLC) remain unknown. In this study, we noted downregulation of miR-501-3p in NSCLC tissues and cell lines. Functional assays showed that overexpression of miR-501-3p suppressed NSCLC cell proliferation, clonogenicity, migration, and invasion. Moreover, miR-501-3p overexpression attenuated in vivo tumor growth in a nude mouse model. In terms of the mechanism, RAP1A was identified as a novel target of miR-501-3p. Overexpression of RAP1A strongly attenuated the inhibitory effects of miR-501-3p on the capacity of NSCLC cells for proliferation and motility. In the clinical samples of NSCLC, miR-501-3p levels negatively correlated with RAP1A expression, which was upregulated in NSCLC. Collectively, these results indicate that miR-501-3p acts as a tumor suppressor in NSCLC by directly targeting RAP1A mRNA and may serve as a theranostic biomarker for patients with NSCLC.

• Authors: Jinchang Lu, Lei Zhou, Bo Wu, Yanhong Duan, Yingxin Sun, Liang Gu, Donghui Xu, Chunling Du
• Journal: Experimental cell research (Exp Cell Res) Vol. 387 Issue 1 Pg. 111752 (02 01 2020) ISSN: 1090-2422 [Electronic] United States
• PMID: 31805277 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 Elsevier Inc. All rights reserved.

Chemical References

• MIRN501 microRNA, human
• MicroRNAs
• RAP1A protein, human
• rap1 GTP-Binding Proteins

Topics

• Animals
• Carcinoma, Non-Small-Cell Lung (genetics)
• Cell Line
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Down-Regulation (genetics)
• Gene Expression Regulation, Neoplastic (genetics)
• Genes, Tumor Suppressor (physiology)
• HEK293 Cells
• Humans
• Lung Neoplasms (genetics)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics)
• Up-Regulation (genetics)
• rap1 GTP-Binding Proteins (genetics)