In this study, a single component
paclitaxel was obtained from fermentation broth by molecular imprinting technique, and its
antiviral, antitumor and immunomodulatory activities were studied. The results showed that
paclitaxel had a good inhibitory activity on human
breast cancer MCF-7 cells and showed a concentration- dependent relationship with an IC50 of about 15 μg/mL in the
sulforhodamine B assay. At the same time,
paclitaxel exerted a weak inhibitory activity on
cervical cancer Hela cells. In addition,
paclitaxel not only inhibited the invasion of HIV-1 pseudovirus into cells, but also exhibited inhibitory activity to a certain extent after viral invasion of the cells. At a
paclitaxel concentration of 20 μg/mL, the inhibition of HIV-1 pseudovirus reached about 66%. The inhibition of
HIV-1 protease activity was concentration-dependent. At a concentration of 20 μg/mL, the inhibitory effect of
paclitaxel on
HIV-1 protease was similar to that of the positive control
pepstatin A, being 15.8%. The
HIV-1 integrase inhibiting activity of
paclitaxel was relatively weak.
Paclitaxel significantly up-regulated the expression of
interleukin-6.