Thermo-reversible injectable hydrogel composing of pluronic F127 and carboxymethyl hexanoyl chitosan for cell-encapsulation.

Abstract

This study demonstrated a novel injectable-thermoreversible hydrogel scaffold composing of PLuronic F127, carboxymethyl hexanoyl chitosan (CA) and glyoxal (Gx) for encapsulating human osteosarcoma MG-63 cells. The hydrogel was prepared by simply mixing CA, F127 and Gx. In so doing, this system exhibited short gelation time and higher gelation temperature. In addition, this hydrogel exhibited thermo-reversibility, that is, the hydrogel can liquefy at room temperature and revert to gel state at body temperature. The encapsulated cells in this hydrogel proliferated more than 400% in the 5-day incubation. Based on these results, these F127/CA/Gx hydrogels can be used to encapsulate cells for tissue engineering applications.

• Authors: Lie-Sian Yap, Ming-Chien Yang
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 185 Pg. 110606 (Jan 01 2020) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 31698265 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Hydrogels
• carboxymethyl-hexanoyl chitosan
• Poloxamer
• Glyoxal
• Chitosan

Topics

• Cell Line, Tumor
• Cell Survival
• Cells, Immobilized (cytology)
• Chitosan (analogs & derivatives, chemistry)
• Glyoxal (chemistry)
• Humans
• Hydrogels (chemistry)
• Injections
• Poloxamer (chemistry)
• Temperature