Detection of fetal trisomy and single gene disease by massively parallel sequencing of extracellular vesicle DNA in maternal plasma: a proof-of-concept validation.

Abstract

BACKGROUND:

During human pregnancy, placental trophectoderm cells release extracellular vesicles (EVs) into maternal circulation. Trophoblasts also give rise to cell-free DNA (cfDNA) in maternal blood, and has been used for noninvasive prenatal screening for chromosomal aneuploidy. We intended to prove the existence of DNA in the EVs (evDNA) of maternal blood, and compared evDNA with plasma cfDNA in terms of genome distribution, fragment length, and the possibility of detecting genetic diseases.

METHODS:

Maternal blood from 20 euploid pregnancies, 9 T21 pregnancies, 3 T18 pregnancies, 1 T13 pregnancy, and 2 pregnancies with FGFR3 mutations were obtained. EVs were separated from maternal plasma, and confirmed by transmission electronic microscopy (TEM), western blotting, and flow cytometry (FACS). evDNA was extracted and its fetal origin was confirmed by quantitative PCR (qPCR). Pair-end (PE) whole genome sequencing was performed to characterize evDNA, and the results were compared with that of cfDNA. The fetal risk of aneuploidy and monogenic diseases was analyzed using the evDNA sequencing data.

RESULTS:

EVs separated from maternal plasma were confirmed with morphology by TEM, and protein markers of CD9, CD63, CD81 as well as the placental specific protein placental alkaline phosphatase (PLAP) were confirmed by western blotting or flow cytometry. EvDNA could be successfully extracted for qPCR and sequencing from the plasma EVs. Sequencing data showed that evDNA span on all 23 pairs of chromosomes and mitochondria, sharing a similar distribution pattern and higher GC content comparing with cfDNA. EvDNA showed shorter fragments yet lower fetal fraction than cfDNA. EvDNA could be used to correctly determine fetal gender, trisomies, and de novo FGFR3 mutations.

CONCLUSIONS:

We proved that fetal DNA could be detected in EVs separated from maternal plasma. EvDNA shared some similar features to plasma cfDNA, and could potentially be used to detect genetic diseases in fetus.

• Authors: Weiting Zhang, Sen Lu, Dandan Pu, Haiping Zhang, Lin Yang, Peng Zeng, Fengxia Su, Zhichao Chen, Mei Guo, Ying Gu, Yanmei Luo, Huamei Hu, Yanping Lu, Fang Chen, Ya Gao
• Journal: BMC medical genomics (BMC Med Genomics) Vol. 12 Issue 1 Pg. 151 (11 04 2019) ISSN: 1755-8794 [Electronic] England
• PMID: 31684971 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cell-Free Nucleic Acids
• DNA
• FGFR3 protein, human
• Receptor, Fibroblast Growth Factor, Type 3

Topics

• Adult
• Aneuploidy
• Cell-Free Nucleic Acids (chemistry, genetics, metabolism)
• DNA (chemistry, genetics, metabolism)
• Down Syndrome (genetics, pathology)
• Extracellular Vesicles (genetics, metabolism)
• Female
• Fetus (metabolism)
• High-Throughput Nucleotide Sequencing (methods)
• Humans
• Pregnancy
• Prenatal Diagnosis
• Receptor, Fibroblast Growth Factor, Type 3 (genetics)
• Sequence Analysis, DNA
• Trisomy