Abstract |
Semen exosomes (SE) inhibit HIV infection. However, the effect of SE on cell activation and inflammation remains unknown. We characterized the response of peripheral blood mononuclear cells (PBMCs) from HIV-uninfected and antiretroviral therapy-suppressed HIV-infected (HIV+) subjects to SE. Quiescent PBMCs or T-cell receptor (TCR)-activated PBMCs from HIV- and HIV+ donors were stimulated with SE in the presence/absence of ex vivo HIV infection. In HIV-infected PBMCs, SE did not reactivate HIV, did not induce lymphoblast development, nor increase CD69+/CD25+ numbers. Furthermore, SE inhibited de novo HIV infection without altering cell activation. SE also asynchronously downregulated HIV-inducible IL1β, IL8, and TNFα and upregulated CXCL10. These data suggest that SE inhibits HIV infection and production of HIV-induced proinflammatory cytokines while preserving lymphocyte activation.
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Authors | Jennifer L Welch, Thomas M Kaufman, Jack T Stapleton, Chioma M Okeoma |
Journal | FEBS letters
(FEBS Lett)
Vol. 594
Issue 4
Pg. 695-709
(02 2020)
ISSN: 1873-3468 [Electronic] England |
PMID | 31665815
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © 2019 Federation of European Biochemical Societies. |
Chemical References |
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Topics |
- Cytokines
(biosynthesis)
- Exosomes
(metabolism)
- Female
- HEK293 Cells
- HIV-1
(physiology)
- Humans
- Inflammation
(metabolism)
- Lymphocyte Activation
- Lymphocytes
(immunology, virology)
- Male
- Semen
(cytology)
- Virus Replication
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