Evaluation of the QuantaMatrix Multiplexed Assay Platform for Molecular Diagnosis of Multidrug- and Extensively Drug-Resistant Tuberculosis Using Clinical Strains Isolated in Myanmar.

Abstract

BACKGROUND:

Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. we developed QuantaMatrix Multiplexed Assay Platform (QMAP) MDR/XDR assay (QuantaMatrix Inc., Seoul, Korea) that can simultaneously detect mutations related to both first- and second-line drug resistance (rifampin, isoniazid, ethambutol, fluoroquinolones, second-line injectable drugs, and streptomycin).

METHODS:

We used 190 clinical Mycobacterium tuberculosis (MTB) strains isolated from Myanmar, compared QMAP and pDST results, and determined concordance rates. Additionally, we performed sequence analyses for discordant results.

RESULTS:

QMAP results were 87.9% (167/190) concordant with pDST results. In the 23 isolates with discordant results, the QMAP and DNA sequencing results completely matched.

CONCLUSIONS:

The QMAP MDR/XDR assay can detect all known DNA mutations associated with drug resistance for both MDR- and XDR-MTB strains. It can be used for molecular diagnosis of MDR- and XDR-TB to rapidly initiate appropriate anti-TB drug therapy.

• Authors: Yunhee Chang, Seoyong Kim, Yeun Kim, Phyu Win Ei, Dasom Hwang, Jongseok Lee, Chulhun L Chang, Hyeyoung Lee
• Journal: Annals of laboratory medicine (Ann Lab Med) Vol. 40 Issue 2 Pg. 142-147 (Mar 2020) ISSN: 2234-3814 [Electronic] Korea (South)
• PMID: 31650730 (Publication Type: Evaluation Study, Journal Article)
• Copyright: © The Korean Society for Laboratory Medicine.

Chemical References

• Antitubercular Agents
• DNA, Bacterial

Topics

• Antitubercular Agents (pharmacology, therapeutic use)
• DNA, Bacterial (genetics, metabolism)
• Drug Resistance, Multiple, Bacterial (genetics)
• Extensively Drug-Resistant Tuberculosis (diagnosis, drug therapy)
• Humans
• Microbial Sensitivity Tests
• Multiplex Polymerase Chain Reaction (methods)
• Mutation
• Myanmar
• Mycobacterium tuberculosis (genetics, isolation & purification)
• Phenotype
• Sequence Analysis, DNA
• Tuberculosis, Multidrug-Resistant (diagnosis, drug therapy)