Investigation of anti-cancer and migrastatic properties of novel curcumin derivatives on breast and ovarian cancer cell lines.

Abstract

BACKGROUND:

Curcumin is known for its multitude of medicinal properties, including anti-cancer and migrastatic activity. Efforts to overcome poor bioavailability, stability, and side effects associated with the higher dose of curcumin has led to the development of newer derivatives of curcumin. Thus, the focus of this study is to screen novel curcumin derivatives, namely ST03 and ST08, which have not been reported before, for their cytotoxicity and migrastatic property on cancer cells.

METHODS:

Anti-cancer activity of ST03 and ST08 was carried out using standard cytotoxicity assays viz., LDH, MTT, and Trypan blue on both solid and liquid cancer types. Flow cytometric assays and western blotting was used to investigate the cell death mechanisms. Transwell migration assay was carried out to check for migrastatic properties of the compounds.

RESULTS:

Both the compounds, ST03 and ST08, showed ~ 100 fold higher potency on liquid and solid tumour cell lines compared to its parent compound curcumin. They induced cytotoxicity by activating the intrinsic pathway of apoptosis in the breast (MDA-MB-231) and ovarian cancer cell lines (PA-1) bearing metastatic and stem cell properties, respectively. Moreover, ST08 also showed inhibition on breast cancer cell migration by inhibiting MMP1 (matrix metalloproteinase 1).

CONCLUSION:

Both ST03 and ST08 exhibit anti-cancer activity at nanomolar concentration. They induce cell death by activating the intrinsic pathway of apoptosis. Also, they inhibit migration of the cancer cells by inhibiting MMP1 in breast cancer cells.

• Authors: Jinsha Koroth, Snehal Nirgude, Shweta Tiwari, Vidya Gopalakrishnan, Raghunandan Mahadeva, Sujeet Kumar, Subhas S Karki, Bibha Choudhary
• Journal: BMC complementary and alternative medicine (BMC Complement Altern Med) Vol. 19 Issue 1 Pg. 273 (Oct 21 2019) ISSN: 1472-6882 [Electronic] England
• PMID: 31638975 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Matrix Metalloproteinase 1
• Curcumin

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Breast Neoplasms (drug therapy, metabolism, physiopathology)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Curcumin (chemistry, pharmacology)
• Drug Screening Assays, Antitumor
• Female
• Humans
• Matrix Metalloproteinase 1 (metabolism)
• Molecular Structure
• Ovarian Neoplasms (drug therapy, metabolism, physiopathology)