Ten-eleven translocation (TET)
enzymes are implicated in DNA demethylation through
dioxygenase activity, which converts
5-methylcytosine to
5-hydroxymethylcytosine (5-hmC). However, the specific roles of TET
enzymes and 5-hmC levels in
head and neck squamous cell carcinoma (
HNSCC) have not yet been evaluated. In this study, we analyzed 5-hmC levels and TET
mRNA expression in a well-characterized dataset of 117 matched pairs of
HNSCC tissues and normal tissues. 5-hmC levels and TET
mRNA expression were examined via
enzyme-linked
immunosorbent assay and quantitative real-time PCR, respectively. 5-hmC levels were evaluated according to various clinical characteristics and prognostic implications. Notably, we found that 5-hmC levels were significantly correlated with
tumor stage (P = 0.032) and recurrence (P = 0.018). Univariate analysis revealed that low levels of 5-hmC were correlated with poor disease-free survival (DFS; log-rank test, P = 0.038). The expression of TET family genes was not associated with outcomes. In multivariate analysis, low levels of 5-hmC were evaluated as a significant independent prognostic factor of DFS (hazard ratio: 2.352, 95% confidence interval: 1.136-4.896; P = 0.021). Taken together, our findings showed that reduction of TET family gene expression and subsequent low levels of 5-hmC may affect the development of
HNSCC.