Noncoding RNAs (ncRNAs), such as
microRNA (
miRNA),
long ncRNA (
lncRNA), and
circular RNA (
circRNA), are regulators of important biological functions. Therefore, understanding their crosstalk and regulatory patterns can provide treatment for diseases. In this study, differentially expressed
RNA transcripts were obtained by
RNA sequencing
in bleomycin-induced
pulmonary fibrosis in mice. Four
miRNAs, 10 lncRNAs, and two
circRNAs were tested to validate the sequencing. There were differentially expressed 585 mRNAs, 236
miRNAs, 272 lncRNAs, and 74
circRNAs in
pulmonary fibrosis. Their location on chromosome, length varieties, interaction, and host genes were analyzed. lnc949, circ949, and circ057 were chosen to explore the detailed crosstalk and regulatory pattern, which were measured by using
RNA-FISH, dual-
luciferase reporter assay, real-time cell analysis and rescue experiment, co-localization analysis,
RNA immunoprecipitation, and
RNA pull down. The data showed that the three ncRNAs were predominant in the cytoplasm, and their regulatory patterns were focused on post-transcription. The fibrotic function of lnc949 depended on its host gene FKBP5. circ949 and circ057 formed a regulatory network with lnc865 and lnc556 to simultaneously regulate miR-29b-2-5p targeting STAT3 phosphorylation. Collectively, different RNAs can crosstalk with each other to regulate
pulmonary fibrosis through different regulatory patterns. We hope these data can provide a full concept of
RNA transcripts, leading to a new treatment for
pulmonary fibrosis.