The detection of
difenidol hydrochloride, which is a
drug that is widely used for treating the
nausea and
vomiting symptoms caused by certain diseases, has been increasingly involved in cases of suicide via overdosing and of
drug poisoning in children. A novel electrochemiluminescence (ECL) sensor for the simple and effective detection of
difenidol hydrochloride was fabricated by modifying a glassy
carbon electrode with three-dimensional
carbon nanofibers (3D-CNFs). The 3D-CNFs were synthesized by electrospinning a mixture of
montmorillonite (
MMT) and
polyacrylonitrile, carbonizing the electrospun product, and etching it with
hydrofluoric acid. The form and structure of the 3D-CNFs was analyzed via scanning electron microscopy, X-ray photoelectron spectroscopy, and Raman microspectroscopy. According to the experimental results obtained using the modified
electrodes, a good linear relationship was found between peak intensity and
difenidol concentration (y = 868.14x - 61.04, R2 = 0.999), with a relatively low detection limit (8.64 × 10-10 mol·L-1 (S/N = 3)). In addition, our approach exhibited good recovery values ranging from 98.99% to 102.28%. The proposed novel ECL sensor has wide application prospects for the detection of
difenidol hydrochloride.