Vibrio harveyi causes severe loss to the aquaculture industry due to its virulence, which is mediated by Quorum sensing (QS) and biofilm formation. In the current study, we have explored the anti-virulent properties and biofilm disruption ability of
luteolin (extracted from coconut shell) and
linalool against this important aquaculture pathogen. HPLC analysis of the methanolic extract of coconut shells revealed a single major peak which matched to the standard
luteolin which was further elucidated by NMR studies. Further,
luteolin and
linalool were screened for their ability to inhibit biofilms and various quorum sensing mediated
virulence factors of V. harveyi. The Minimum Inhibitory Concentration (MIC) of the two compounds was determined and the sub-inhibitory concentrations of the compounds were able to inhibit biofilm formation. Both the compounds disrupted about 60-70% mature biofilms, which was also visually observed by light microscopy. Both
linalool and
luteolin exhibited a significant reduction in the production of EPS and
alginate in the biofilms matrix of V. harveyi which was confirmed by Scanning Electron Microscopy (SEM). Both compounds inhibited the swarming and swimming motility, the crucial quorum sensing (QS) mediated virulence of V. harveyi. The present study shows the presence of valuable polyphenolic compound like
luteolin in coconut shells that are discarded as a waste. From the present study we envisage that
luteolin and
linalool can serve as potent anti-virulent agents to combat QS mediated
infections against aquaculture pathogens.