Abstract | PURPOSE: To determine whether combinations of commonly used antiamoebic agents display synergy in their ability to kill Acanthamoeba cysts in vitro. METHODS: Synergy testing was performed with a microdilution checkerboard assay on 10 clinical Acanthamoeba keratitis isolates collected at the Proctor Foundation from 2008 to 2012. Each isolate was exposed to pairwise combinations of chlorhexidine, propamidine, and voriconazole. The minimum cysticidal concentration (MCC) for each drug pair was estimated for each isolate, and the summed fractional cysticidal concentration (ΣFCC) was calculated for each drug combination in the checkerboard, with synergy defined as a lack of growth at a ΣFCC ≤ 0.5 and antagonism as growth at a ΣFCC > 4. RESULTS: CONCLUSIONS: In an in vitro assay, voriconazole reduced the cysticidal activity of 2 commonly used antiamoebic drugs. Although the in vivo drug interactions could be different, these observations may be useful in cases of nonhealing Acanthamoeba keratitis being treated with combination therapies that include voriconazole.
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Authors | Maya Talbott, Vicky Cevallos, Michael C Chen, Stephanie A Chin, Prajna Lalitha, Gerami D Seitzman, Thomas M Lietman, Jeremy D Keenan |
Journal | Cornea
(Cornea)
Vol. 38
Issue 10
Pg. 1309-1313
(Oct 2019)
ISSN: 1536-4798 [Electronic] United States |
PMID | 31306283
(Publication Type: Journal Article)
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Chemical References |
- Amebicides
- Antifungal Agents
- Voriconazole
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Topics |
- Acanthamoeba
(drug effects, isolation & purification)
- Acanthamoeba Keratitis
(diagnosis, drug therapy, parasitology)
- Amebicides
(pharmacology)
- Animals
- Antifungal Agents
(pharmacology)
- Drug Synergism
- Eye Infections, Parasitic
(drug therapy, parasitology)
- Humans
- Parasitic Sensitivity Tests
- Voriconazole
(pharmacology)
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