Long non-coding RNAs (lncRNAs) have shown critical roles in multiple
cancers via competitively binding common
microRNAs. miR-214 has been proved to play tumour suppressive roles in various
cancers, including
cervical cancer. In this study, we identified that
lncRNA LINC01535 physically binds miR-214, relieves the repressive roles of miR-214 on its target EZH2, and therefore up-regulates EZH2
protein expression. Intriguingly, we also found that EZH2 directly represses the expression of miR-214. Thus, miR-214 and EZH2 form double negative regulatory loop. Through up-regulating EZH2, LINC01535 further represses miR-214 expression. Functional experiments showed that enhanced expression of LINC01535 promotes
cervical cancer cell growth, migration and invasion in vitro and
cervical cancer xenograft growth in vivo. Reciprocally, LINC01535 knockdown suppresses
cervical cancer cell growth, migration and invasion. Activation of the miR-214/EZH2 regulatory loop by overexpression of miR-214 or silencing of EZH2 reverses the roles of LINC01535 in promoting cervical canc`er cell growth, migration and invasion in vitro and
cervical cancer xenograft growth in vivo. Clinically, LINC01535 is significantly up-regulated in
cervical cancer tissues and correlated with advanced clinical stage and poor prognosis. Moreover, the expression of LINC01535 is reversely associated with the expression of miR-214 and positively associated with the expression of EZH2 in
cervical cancer tissues. In conclusion, this study reveals that LINC01535 promotes
cervical cancer progression via repressing the miR-214/EZH2 regulatory loop.