Biological roles of filamin a in prostate cancer cells.

Abstract

OBJECTIVE:

This study aims to investigate the association of filamin A with the function and morphology of prostate cancer (PCa) cells, and explore the role of filamin A in the development of PCa, in order to analyze its significance in the evolvement of PCa.

MATERIALS AND METHODS:

A stably transfected cell line, in which filamin A expression was suppressed by RNA interference, was first established. Then, the effects of the suppression of filamin A gene expression on the biological characteristics of human PCa LNCaP cells were observed through cell morphology, in vitro cell growth curve, soft agar cloning assay, and scratch test.

RESULTS:

A cell line model with a low expression of filamin A was successfully constructed on the basis of LNCaP cells. The morphology of cells transfected with plasmid pSilencer-filamin A was the following: Cells were loosely arranged, had less connection with each other, had fewer tentacles, and presented a fibrous look. The growth rate of LNCap cells was faster than cells transfected with plasmid pSilencer-filamin A (P<0.05). The clones of LNCap cells in the soft agar cloning assay was significantly fewer than that of cells stably transfected with plasmid pSilencer-filamin A (P<0.05). Cells stably transfected with plasmid pSilencer-filamin A presented with a stronger healing and migration ability compared to LNCap cells (healing rate was 32.2% and 12.1%, respectively; P<0.05).

CONCLUSION:

The expression of the filamin A gene inhibited the malignant development of LNCap cells. Therefore, the filamin A gene may be a tumor suppressor gene.

• Authors: Xue-Chao Li, Chuan-Xi Huang, Shi-Kui Wu, Lan Yu, Guang-Jian Zhou, Li-Jun Chen
• Journal: International braz j urol : official journal of the Brazilian Society of Urology (Int Braz J Urol) 2019 Sep-Oct Vol. 45 Issue 5 Pg. 916-924 ISSN: 1677-6119 [Electronic] Brazil
• PMID: 31268639 (Publication Type: Journal Article)
• Copyright: Copyright® by the International Brazilian Journal of Urology.

Chemical References

• Filamins
• Formazans
• Tetrazolium Salts
• MTT formazan

Topics

• Blotting, Western
• Cell Line, Tumor
• Cell Proliferation
• Cells, Cultured
• Colorimetry (methods)
• Filamins (analysis, genetics, physiology)
• Formazans
• Humans
• Male
• Plasmids
• Prostatic Neoplasms (genetics, pathology)
• Tetrazolium Salts
• Time Factors
• Transfection (methods)
• Wound Healing (physiology)