Study of 5-Fluorouracil Loaded Chitosan Nanoparticles for Treatment of Skin Cancer.

Abstract

BACKGROUND:

The purpose of this study was to formulate, characterize and in-vitro cytotoxicity of 5-Fluorouracil loaded controlled release nanoparticles for the treatment of skin cancer. The patents on nanoparticles (US8414926B1), (US61654404A), (WO2007150075A3) etc. helped in the selection polymers and method for the preparation of nanoparticles.

METHODS:

In the present study nanoparticles were prepared by simple ionic gelation method using various concentrations of chitosan and sodium tripolyphosphate (TPP). Several process and formulation parameters were screened and optimized using 25-2 fractional factorial design. The prepared nanoparticles were evaluated for particle size, shape, charge, entrapment efficiency, crosslinking mechanism and drug release study.

RESULTS:

The optimized 5-Fluorouracil loaded nanoparticle were found with particle size of of 320±2.1 nm, entrapment efficiency of 85.12%± 1.1% and Zeta potential of 29mv±1mv. Scanning electron microscopy and dynamic light scattering technique revealed spherical particles with uniform size. The invitro release profile showed controlled release up to 24 hr. Further study was carried using A375 basal cell carcinoma cell-line to elucidate the mechanism of its cytotoxicity by MTT assay.

CONCLUSION:

These results demonstrate that the possibility of delivering 5-Fluorouracil to skin with enhanced encapsulation efficiency indicating effectiveness of the formulation for treatment of basal cell carcinoma type of skin cancer.

• Authors: Gayatri Patel, Bindu K N Yadav
• Journal: Recent patents on nanotechnology (Recent Pat Nanotechnol) Vol. 14 Issue 3 Pg. 210-224 ( 2020) ISSN: 2212-4020 [Electronic] United Arab Emirates
• PMID: 31267881 (Publication Type: Journal Article)
• Copyright: Copyright© Bentham Science Publishers; For any queries, please email at [email protected].

Chemical References

• Polyphosphates
• Chitosan
• triphosphoric acid
• Fluorouracil

Topics

• Analysis of Variance
• Calorimetry, Differential Scanning
• Cell Death (drug effects)
• Cell Line, Tumor
• Chitosan (chemistry)
• Drug Compounding
• Drug Liberation
• Fluorouracil (pharmacology, therapeutic use)
• Humans
• Nanoparticles (chemistry, ultrastructure)
• Particle Size
• Polyphosphates (chemistry)
• Regression Analysis
• Skin Neoplasms (drug therapy, pathology)
• Spectroscopy, Fourier Transform Infrared
• Static Electricity
• Thermogravimetry
• Time Factors